Protective effect of naringin in rat model of renal ischemia reperfusion injury

Mutlu Deger Department of Urology, Faculty of Medicine, Cukurova University, Adana, Turkey
Nebil Akdogan Department of Urology, Faculty of Medicine, Cukurova University, Adana, Turkey
Volkan Izol Department of Urology, Faculty of Medicine, Cukurova University, Adana, Turkey
Halil Mahir Kaplan Department of Pharmacology, Faculty of Medicine, Cukurova University, Adana, Turkey
Perçin Pazarci Department of Medical Biology, Faculty of Medicine, Cukurova University, Adana, Turkey
Ibrahim Atilla Arıdogan Department of Urology, Faculty of Medicine, Cukurova University, Adana, Turkey

Keywords: renal ischaemia reperfusion injury; naringin; inflammation; apoptosis

Abstract

Objective: We aimed to research that naringin whether protects from renal ischemia/reperfusion induced renal damage in rats. Methods: Twenty-four Wistar albino female rats randomly were divided into three groups: 1) control group, in which the rats were only performed right nephrectomy; 2) a second group received right nephrectomy and left kidney ischemia (1 h) and reperfusion (24 h) group ischemia/reperfusion (I/R); 3) a third group received 50 mg/kg naringin orally once a day for two weeks before ischemia/reperfusion (I/R/N). Expression of cyclooxygenase-2 (COX-2), cytosolic phospholipase A₂ (cPLA2), inducible nitric oxide synthase (iNOS), caspase-3, B-cell lymphoma-2 (Bcl-2), Bcl-2 associated x protein (Bax), serum creatinine (Cr), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) were measured by using enzyme-linked immunosorbent assay (ELISA). Results: Naringin-treated rats that performed renal ischemia/reperfusion demonstrated significant decrease in Cr, IL-6 and TNF-α levels when compared to the only renal ischemia/reperfusion performed rats. While renal ischemia/reperfusion caused a decrease of bcl-2 (1.72 ± 0.20 pg/ml) levels, while an increase of COX-2 (11882 ± 642 pg/ml), cPLA2 (2448 ± 139 pg/ml), iNOS (4331 ± 438 IU/ml), cleaved caspase-3 (7.33 ± 0.76 ng/ml) and Bax (2.33 ± 0.44 ng/ml) levels. The treatment of naringin reversed these kidney effects (7.47 ± 60.35 pg/ml; 9299 ± 327 pg/ml; 2001 ± 78 pg/ml; 3112 ± 220 IU/ml; 3.38 ± 0.54 ng/ml; 2.33 ± 0.44 ng/ml, respectively) (p <0.05). Conclusion: This study showed that naringin treatment attenuated renal damage induced by ischemia/reperfusion in rats.

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Published

2021-06-22

How to Cite

Deger M, Akdogan N, Izol V, Kaplan HM, Pazarci P, Arıdogan IA. Protective effect of naringin in rat model of renal ischemia reperfusion injury. Rev Nefrol Dial Traspl. [Internet]. 2021Jun.22 [cited 2024Dec.27];41(2):113-8. Available from: http://vps-1689312-x.dattaweb.com/index.php/rndt/article/view/647

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Vol. 41 No. 2 (2021): Abr.-Jun.

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